Affiliation:
1. Department of Cell and Developmental Biology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom
Abstract
Abstract
Isolated mesophyll cells from Zinnia elegans are induced by auxin and cytokinin to form tracheary elements (TEs) in vitro with high synchrony. To reveal the changing patterns of gene expression during the 48 h of transdifferentiation from meso-phyll to TE cell fate, we used a cDNA–amplified fragment length polymorphism approach to generate expression profiles of >30,000 cDNA fragments. Transcriptional changes of 652 cDNA fragments were observed, of which 304 have no previously described function or sequence identity. Sixty-eight genes were upregulated within 30 min of induction and represent key candidates for the processes that underlie the early stages of commitment and differentiation to a TE cell fate.
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science
Reference42 articles.
1. Allona, I., Quinn, M., Shoop, E., Swope, K., St. Cyr, S., and Carlis, J. (1998). Analysis of xylem formation in pine by cDNA sequencing. Proc. Natl. Acad. Sci. USA
95
,
9693–9698.
2. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J.H., Zhang, Z., Miller, W., and Lipman, D.J. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res.
25
,
3389–3402.
3. Arabidopsis Genome Initiative (2000). Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature
408
,
796–815.
4. Azmi, A., Dewitte, W., Van Onckelen, H., and Chriqui, D. (2001). In situ localization of endogenous cytokinins during shooty tumor development on Eucalyptus globulus Labill. Planta
213
,
29–36.
5. Bachem, C.W.B., van der Hoeven, R.S., de Bruijn, S.M., Vreugdenhil, D., Zabeau, M., and Visser, R.G.F. (1996). Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development. Plant J.
9
,
745–753.
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