Expression profile of plasma microRNAs and target genes in patients with complicated pregnancy-Reference-Cited by-同舟云学术

Expression profile of plasma microRNAs and target genes in patients with complicated pregnancy

Published:2023-06-27 Issue:3 Volume:17 Page:309-320
ISSN:2500-3194
Container-title:Obstetrics, Gynecology and Reproduction
language:
Short-container-title:Akušerstvo, ginekologiâ i reprodukciâ

Author:

Umerova M. D.¹^ORCID,Alyadinova S. S.¹^ORCID,Khonjonova M. J.¹^ORCID,Balakadasheva R. A.¹^ORCID,Menadzhiev E. E.¹^ORCID,Kharchuk D. I.¹^ORCID,Leus D. O.¹^ORCID,Islyamova G. I.¹^ORCID,Islyamova M. I.¹^ORCID,Abkarimova S. L.²^ORCID,Kasabyan D. S.²^ORCID,Sorokina L. E.³^ORCID

Affiliation:

1. Institute "Georgievsky Medical Academy", Vernadsky Crimean Federal University

2. Kuban State University

3. National Research Center – Institute of Immunology, Federal Medical-Biological Agency of Russia

Abstract

Aim: to assess features of placental transcriptome in patients with complicated pregnancy course and outcome.Materials and Methods. A prospective observational comparative study in parallel groups was carried out by examining 44 patients divided into three groups: group 1 included 13 pregnant women with moderate and severe preeclampsia (PE), group 2 consisted of 12 patients with fetal growth retardation (FGR), control group included 19 clinically healthy women with uncomplicated pregnancy and delivery. All women enrolled to the study underwent a comprehensive examination, including history collection, general and obstetric-gynecological examination, laboratory and instrumental studies. Special research methods were used during the study, which included placental tissue sampling followed by analyzed expression profile of 12 microRNAs using real-time polymerase chain reaction (PCR).Results. The PCR data indicated about lack of expression for analyzed miR-210-3p, miR-320-3p, miR-1304-5p and miR-375-5p in placental tissue samples in FGR patients. No significant differences in placental miRNA levels were observed in FGR vs. PE and control group. Analysis of placenta-specific microRNAs in women with PE vs. control group showed a significantly down modulated expression level for miR-517a-3p (p = 0.025), miR-517c-3p (p = 0.036), miR-574-5p (p = 0.015) along with upregulated expression of miR-20a-5p (p = 0.046).Conclusion. The data obtained evidence that pregnancy-related complications are characterized by specific molecular changes at placental transcriptome level.

Publisher

IRBIS

Subject

Obstetrics and Gynecology,Embryology,Reproductive Medicine

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