A Chromosome Bin Map of 16,000 Expressed Sequence Tag Loci and Distribution of Genes Among the Three Genomes of Polyploid Wheat-Reference-Cited by-同舟云学术

A Chromosome Bin Map of 16,000 Expressed Sequence Tag Loci and Distribution of Genes Among the Three Genomes of Polyploid Wheat

Published:2004-10-01 Issue:2 Volume:168 Page:701-712
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Qi L L¹,Echalier B¹,Chao S²,Lazo G R³,Butler G E⁴,Anderson O D³,Akhunov E D⁵,Dvořák J⁵,Linkiewicz A M⁵,Ratnasiri A⁵,Dubcovsky J⁵,Bermudez-Kandianis C E⁶,Greene R A⁶,Kantety R⁶,La Rota C M⁶,Munkvold J D⁶,Sorrells S F⁶,Sorrells M E⁶,Dilbirligi M⁷,Sidhu D⁷,Erayman M⁷,Randhawa H S⁷,Sandhu D⁷,Bondareva S N⁷,Gill K S⁷,Mahmoud A A⁸,Ma X-F⁸,Gustafson J P⁹,Conley E J¹⁰,Nduati V¹⁰,Gonzalez-Hernandez J L¹⁰,Anderson J A¹⁰,Peng J H¹¹,Lapitan N L V¹¹,Hossain K G¹²,Kalavacharla V¹²,Kianian S F¹²,Pathan M S⁸,Zhang D S¹³,Nguyen H T⁸,Choi D-W¹⁴,Fenton R D¹⁴,Close T J¹⁴,McGuire P E²,Qualset C O²,Gill B S¹,

Affiliation:

1. Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, Manhattan, Kansas 66506-5502

2. Genetic Resources Conservation Program, University of California, Davis, California 95616

3. USDA-ARS Western Regional Research Center, Albany, California 94710

4. Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721

5. Department of Agronomy and Range Science, University of California, Davis, California 95616

6. Department of Plant Breeding, Cornell University, Ithaca, New York 14853

7. Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164-6420

8. Department of Agronomy, University of Missouri, Columbia, Missouri 65211

9. USDA-ARS Plant Genetics Research Unit, Department of Agronomy, University of Missouri, Columbia, Missouri 65211

10. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108

11. Department of Soil and Crop Sciences, Colorado State University, Fort Collins, Colorado 80523-1170

12. Department of Plant Sciences, North Dakota State University, Fargo, North Dakota 58105-5051

13. Department of Plant and Soil Science, Texas Tech University, Lubbock, Texas 79409

14. Department of Botany and Plant Sciences, University of California, Riverside, California 92521

Abstract

Abstract Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/168/2/701/42060044/genetics0701.pdf

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