13C metabolic flux profiling ofPichia pastorisgrown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids

Author:

Zhang Meng1,Yu Xiao-Wei1,Xu Yan1,Jouhten Paula2,Swapna Gurla V. T.3,Glaser Ralf W.4,Hunt John F.5,Montelione Gaetano T.36,Maaheimo Hannu7,Szyperski Thomas8

Affiliation:

1. School of Biotechnology; Key Laboratory of Industrial Biotechnology; State Key Laboratory of Food Science and Technology; Ministry of Education; Jiangnan University; Wuxi China

2. European Molecular Biology Laboratory Heidelberg; Germany

3. Department of Molecular Biology and Biochemistry; Center for Advanced Biotechnology and Medicine; Rutgers, The State University of New Jersey; Piscataway NJ USA

4. Institute of Biochemistry and Biophysics; Friedrich-Schiller-Universität; Jena Germany

5. Department of Biological Sciences; Columbia University; New York NY USA

6. Department of Biochemistry and Molecular Biology; Robert Wood Johnson Medical School; Rutgers, The State University of New Jersey; Piscataway NJ USA

7. VTT Technical Research Centre of Finland Ltd; Espoo Finland

8. Department of Chemistry; State University of New York at Buffalo; NY USA

Funder

High-end Foreign Experts Recruitment Program

National Natural Science Foundation of China

Six Talent Peaks Project in Jiangsu Province

333 Project in Jiangsu Province

111 Project

National Institutes of Health

Jerome and Lorraine Aresty Chair Endowment

Publisher

Wiley

Subject

Cell Biology,Molecular Biology,Biochemistry

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