Transcriptome reveals BCAAs biosynthesis pathway is influenced by lovastatin and can act as a potential control target in Phytophthora sojae
Author:
Yang Xinyu1ORCID,
Huang Qifeng1,
Xu Jitao1,
Gao Zhen1,
Jiang Xue1,
Wu Yuanhua1,
Ye Wenwu2,
Liang Yue1
Affiliation:
1. College of Plant Protection Shenyang Agricultural University Shenyang Liaoning China
2. College of Plant Protection Nanjing Agricultural University Nanjing Jiangsu China
Abstract
Abstract
Aims
Lovastatin has been indicated to impair growth and development of Phytophthora sojae. Therefore, this study was performed to understand the inhibitory mechanism of lovastatin and investigate the metabolic pathway potentially served as a new control target for this plant pathogen.
Methods and Results
Whole transcriptome analysis of lovastatin-treated P. sojae was performed by RNA-sequencing. The results revealed that 84 genes were upregulated and 58 were downregulated with more than fourfold changes under treatment. Kyoto Encyclopaedia of Genes and Genomes analysis indicated that the branched-chain amino acids (BCAAs) biosynthesis pathway was abundantly enriched. All enzymes in the BCAAs biosynthesis pathway were identified in the P. sojae genome. Moreover, the study found that the herbicide flumetsulam targeting acetohydroxyacid synthase (AHAS) of the BCAAs biosynthesis pathway could effectively inhibit mycelial growth of P. sojae.
Conclusions
Lovastatin treatment significantly influences the BCAAs biosynthesis pathway in P. sojae. Moreover, the herbicide flumetsulam targets AHAS and inhibits growth of P. sojae.
Significance and Impact of the Study
The present study revealed that BCAAs biosynthesis pathway was influenced by lovastatin treatment and its key enzyme AHAS was identified as a potential new control target, which provides clues for exploring more oomycetes to control plant diseases caused by P. sojae.
Funder
Introduced Talents of Shenyang Agricultural University
National Natural Science Foundation of China
Scientific Research Foundation of the Educational Department of Liaoning Province
Starting Grants of Shenyang Agricultural University
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Cited by
1 articles.
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