Affiliation:
1. Department of Medicine, King’s College School of Medicine and Dentistry, London, UK
Abstract
SUMMARY
The effects of immunoglobulin preparations from hyperthyroid Graves’ disease patients on primary cultures of thyroid cells have been studied at the mRNA level. Autoantibodies to the thyrotropin (TSH) receptor from these patients, which had been initially characterized by their ability to stimulate adenylale cyclase and inhibit the binding of radiolabelled TSH to thyroid membrane preparations, were studied for their effects on thyroglobulin and thyroid peroxidase mRNA levels. Incubation of thyroid cells with TSH receptor autoantibodies from different Graves'disease patients for 48 h led to time- and dose-dependent increases in the levels of thyroid peroxidase and thyroglobulin mRNA in primary cultures of thyrocytes. The incomplete correlation between G protein-linked adenylate cyclase activation and thyroid mRNA elevation indicates the possibility of the involvement of alternative second messenger pathways in the regulation of thyroid cell function and differentiation.
Publisher
Oxford University Press (OUP)
Subject
Immunology,Immunology and Allergy
Reference13 articles.
1. Immunoiogical and molecular characteristics of the thyroid peroxidase autoantigen;Samon;Autommunity,1991
2. Quantitative estimation of specific mRNA levels in cultured cells using gene screen plus membrane;Banga;Biotechnol Update,1990
3. Characterization of the cyclic AMP response to thyrotropin in monolayer cultures of normal human thyroid cells;Bidey;Acta Endorinal,1981
4. Single-step method of RNA isolation by acid guanidinium thyoeyanate-phenol-chloroform exlraction;Chomczynski;Anal. Biochem,1987
5. Activation of the thyroid peroxidase gene in human thyroid cells: cited of thyrotrophin. forskolin and phorbol ester;Collison;J. Molec. Endocrinol,1989
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献