Long non‐coding RNA H19 mediates osteogenic differentiation of bone marrow mesenchymal stem cells through the miR‐29b‐3p/DKK1 axis

Abstract

AbstractSingle immobilization theory cannot fully account for the extensive bone loss observed after spinal cord injury (SCI). Bone marrow mesenchymal stem cells (BMSCs) are crucial in bone homeostasis because they possess self‐renewal capabilities and various types of differentiation potential. This study aimed to explore the molecular mechanism of long non‐coding RNA H19 in osteoporosis after SCI and provide new research directions for existing prevention strategies. We used small interfering RNA to knockdown H19 expression and regulated miR‐29b‐2p expression using miR‐29b‐3p mimetics and inhibitors. Western blotting, real‐time fluorescence quantitative PCR, Alizarin red staining, alkaline phosphatase staining and double‐luciferase reporter gene assays were used to assess gene expression, osteogenic ability and binding sites. lncRNA H19 was upregulated in BMSCs from the osteoporosis group, whereas miR‐29b‐3p was downregulated. We identified the binding sites between miR‐29b‐3p and lncRNAs H19 and DKK1. H19 knockdown promoted BMSCs' osteogenic differentiation, whereas miR‐29b‐3p inhibition attenuated this effect. We discovered potential binding sites for miR‐29b‐3p in lncRNAs H19 and DKK1. Our findings suggest that long non‐coding RNA H19 mediates BMSCs' osteogenic differentiation in osteoporosis after SCI through the miR‐29b‐3p/DKK1 axis and by directly inhibiting the β‐catenin signalling pathway.