Beyond the phenotype: Exploring inherited retinal diseases with targeted next‐generation sequencing in a Turkish cohort

Author:

Ozguc Caliskan Busra1ORCID,Uslu Kubra1,Sinim Kahraman Neslihan2ORCID,Erkilic Kuddusi3,Oner Ayse2ORCID,Dundar Munis1ORCID

Affiliation:

1. Faculty of Medicine, Department of Medical Genetics Erciyes University Kayseri Turkey

2. Department of Ophthalmology Acibadem Kayseri Hospital Kayseri Turkey

3. Faculty of Medicine, Department of Ophthalmology Erciyes University Kayseri Turkey

Abstract

AbstractThis research aims to compile recent clinical and genetic data from Turkish patients with inherited retinal disorders and evaluate the effectiveness of targeted Next‐generation sequencing panels. The study included Turkish individuals with hereditary retinal diseases who visited the Medical Genetic Department of Erciyes University between 2019 and 2022. One proband per family was selected based on eligibility. We used Hereditary Disorder Solution (HDS) by Sophia Genetics and performed next‐generation sequencing (NGS) with Illumina NextSeq‐500. Bioinformatics analysis using Sophia DDM® SaaS algorithms and ACMG guidelines classified genomic changes. The study involved 354 probands. Disease‐causing variants were found in 58.1% of patients, with ABCA4, USH2A, RDH12, and EYS being the most frequently implicated genes. Forty‐eight novel variants were detected. This study enhances the knowledge of clinical diagnoses, symptom onset, inheritance patterns, and genetic details for Turkish individuals with hereditary retinal disease. It contributes to broader health strategies by enabling comparisons with other studies.

Publisher

Wiley

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