Affiliation:
1. State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences Henan University Kaifeng 475004 China
2. Academy for Advanced Interdisciplinary Studies Henan University Kaifeng 475004 China
Abstract
Summary
Cyanobacterial blooms pose a serious threat to public health due to the presence of cyanotoxins. Microcystin‐LR (MC‐LR) produced by Microcystis aeruginosa is the most common cyanotoxins. Due to the limitation of isolation, purification, and genetic manipulation techniques, it is difficult to study and verify in situ the biosynthetic pathways and molecular mechanisms of MC‐LR.
We reassembled the biosynthetic gene cluster (mcy cluster) of MC‐LR in vitro by synthetic biology, designed and constructed the strong bidirectional promoter biPpsbA2, transformed it into Synechococcus 7942, and successfully expressed MC‐LR at a level of 0.006–0.018 fg cell−1 d−1.
We found the expression of MC‐LR led to abnormal cell division and cellular filamentation, further using various methods proved that by irreversibly competing its GTP‐binding site, MC‐LR inhibits assembly of the cell division protein FtsZ.
The study represents the first reconstitution and expression of the mcy cluster and the autotrophic production of MC‐LR in model cyanobacterium, which lays the foundation for resolving the microcystins biosynthesis pathway. The discovered role of MC‐LR in cell division reveals a mechanism of how blooming cyanobacteria gain a competitive edge over their nonblooming counterparts.
Funder
Higher Education Discipline Innovation Project
National Key Research and Development Program of China
National Natural Science Foundation of China
Natural Science Foundation of Henan Province
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献