Accuracy of RHC/c genotyping in Chinese Han population

Abstract

AbstractBackground and ObjectivesThe RHCE gene plays an important role in the complex and polymorphic Rh blood group system. RHCE genotyping holds significant clinical and transfusion‐related implications. The objective of this study was to evaluate the accuracy of RHC/c genotyping in the Chinese Han population.Materials and MethodsBlood samples were obtained from 653 Chinese Han blood donors. The serological RhD and RhCcEe types were determined using monoclonal antibodies. Subsequently, multiplex real‐time polymerase chain reaction (PCR) analysis was performed for RHC and RHc genotyping. Additionally, exon 2 of RHCE and exon 1 of RHD were sequenced.ResultsThe analysis in this study found 443 RhD‐positive donors and 210 RhD‐negative donors. Among the 653 total donors, discrepancies between the RHC genotyping results and the serological results were found in 37 individuals. Specifically, 6 false‐positive RhC results in RhD‐positive donors and 28 false‐positive RhC results in RhD‐negative donors were identified based on c.48C in RHCE exon 1. Additionally, 3 false‐negative RhC results were observed in the RhD‐positive donors due to a 109 bp insertion in RHCE intron 2. RHc typing demonstrated complete consistency between the real‐time PCR and the serological results.ConclusionIn the Chinese Han population, RHC genotyping was reliable when consistent results were achieved by both c.48C‐based and 109 bp insertion‐based genotyping. Moreover, RHc genotyping based on c.203A and c.307C polymorphic loci demonstrated dependable performance.