Affiliation:
1. Department of Medical Biology, Medicine Faculty Karamanoğlu Mehmetbey University Karaman Turkey
2. Department of Pediatric Immunology and Allergy, Medicine Faculty Necmettin Erbakan University Konya Turkey
3. Department of Medical Genetic, Medicine Faculty KTO Karatay University Konya Turkey
Abstract
CD19 deficiency is a rare, predominantly antibody deficiency, and there are few studies showing that it can be seen in autoimmune diseases. The aim of study was evaluated to transcription factor and cytokine expressions of helper T (Th)‐cell subsets in CD19 deficiency and the possible mechanism role of this factor expression in autoimmune disease. Transcription factor and cytokine expressions of Th1, Th2, Th17, and regulatory T (Treg) cells were investigated by real‐time polymerase chain reaction (qPCR) method. In the study, in the patient/control comparison, transcription factor and cytokine expressions of Th1 (T‐bet, STAT1, and STAT4) were found to be significantly downregulated, but IFN‐γ was significantly upregulated in patients. Th2 factor GATA3, STAT6, IL‐4, and IL‐5 were significantly downregulated. For Th17, RORγt was downregulated while IL‐22 was upregulated. In the heterozygous/control comparison, there was no significant change in gene expressions other than IL‐5. T‐bet, STAT1, GATA3, IL‐4, RORγt, FoxP3, and TGF‐β were significantly downregulated in the patient/heterozygous comparison. It was revealed for the first time that the expression of the transcription factors and cytokines in CD19 deficiency. These findings might be showing the predominance of Th1 factors and suppressed Treg factors which could be related with autoimmunity in CD19 deficiency.
Subject
Microbiology (medical),General Medicine,Immunology and Allergy,Pathology and Forensic Medicine
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献