Coordination of miR319–TaPCF8 with TaSPL14 orchestrates auxin signaling and biosynthesis to regulate plant height in common wheat

Author:

Hao Pingan12ORCID,Jian Chao2ORCID,Hao Chenyang2ORCID,Liu Shujuan2ORCID,Hou Jian2ORCID,Liu Hongxia2ORCID,Liu Haixia2ORCID,Zhang Xueyong2ORCID,Zhao Huixian1ORCID,Li Tian2ORCID

Affiliation:

1. College of Life Sciences Northwest A&F University Yangling 712100 China

2. State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China

Abstract

ABSTRACTWheat culms, comprising four to six internodes, are critically involved in determining plant height and lodging resistance, essential factors for field performance and regional adaptability. This study revealed the regulatory function of miR319 in common wheat plant height. Repression of tae‐miR319 through short tandem target mimics (STTM) caused an increased plant height, while overexpression (OE) of tae‐miR319 had the opposite effect. Overexpressing a miR319‐resistant target gene TaPCF8 (rTaPCF8), increased plant height. TaPCF8 acted as a transcription repressor of downstream genes TaIAAs, which interact physically with TaSPL14. The significant differences of indole‐3‐acetic acid (IAA) contents indicate the involvement of auxin pathway in miR319‐mediated plant height regulation. Finally, we identified two TaPCF8 haplotypes in global wheat collections. TaPCF8‐5A‐Hap2, as per association and evolution examinations, was subjected to strong substantial selection throughout wheat breeding. This haplotype, associated with shorter plant height, aligns with global breeding requirements. Consequently, in high‐yield wheat breeding, we proposed a potential molecular marker for marker‐assisted selection (MAS). Our findings offer fresh perspectives into the molecular mechanisms that underlie the miR319–TaPCF8 module's regulation of plant height by orchestrating auxin signaling and biosynthesis in wheat.

Funder

National Natural Science Foundation of China

Publisher

Wiley

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