Affiliation:
1. State Key Laboratory of Animal Nutrition, Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing China
Abstract
AbstractSerine proteases are among the most important biological additives in various industries such as detergents, leather, animal feed and food. A serine protease gene,Fgapt4, fromFusarium graminearum2697 was identified, cloned and expressed inPichia pastoris. The optimal pH and temperature ofFgAPT4 were 8.5 and 40°C, respectively. The relative activity was >30% even at 10°C. It had a wide range of pH stability (4.0–12.0) and detergent compatibility. To improve the catalytic activity, a strategy combining molecular docking and evolutionary analysis was adopted. Twelve amino acid residue sites and three loops (A, B and C) were selected as potential hot spots that might play critical roles in the enzyme's functional properties. Twenty‐eight mutants targeting changes in individual sites or loops were designed, and mutations with good performance were combined. The best mutant wasFgAPT4‐M3 (Q70N/D142S/A143S/loop C). The specific activity and catalytic efficiency ofFgAPT4‐M3 increased by 1.6 (1008.5 vs. 385.9 U/mg) and 2.2‐fold (3565.1 vs. 1106.3/s/mM), respectively. Computational analyses showed that the greater flexibility of the substrate pocket may be responsible for the increased catalytic activity. In addition, its application in detergents indicated thatFgAPT4‐M3 has great potential in washing.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Subject
Applied Microbiology and Biotechnology,Biochemistry,Bioengineering,Biotechnology
Cited by
8 articles.
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