A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1

Author:

van Es Sam W.12ORCID,Muñoz‐Gasca Aitor3ORCID,Romero‐Campero Francisco J.45ORCID,González‐Grandío Eduardo3ORCID,de los Reyes Pedro4ORCID,Tarancón Carlos3,van Dijk Aalt D. J.6ORCID,van Esse Wilma12ORCID,Pascual‐García Alberto7ORCID,Angenent Gerco C.12ORCID,Immink Richard G. H.12ORCID,Cubas Pilar3ORCID

Affiliation:

1. Bioscience, Wageningen Plant Research Wageningen University & Research 6708 PB Wageningen the Netherlands

2. Laboratory of Molecular Biology Wageningen University & Research 6708 PB Wageningen the Netherlands

3. Department of Plant Molecular Genetics Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma de Madrid 28049 Madrid Spain

4. Institute for Plant Biochemistry and Photosynthesis Universidad de Sevilla – Consejo Superior de Investigaciones Científicas Ave. Américo Vespucio 49 41092 Seville Spain

5. Department of Computer Science and Artificial Intelligence Universidad de Sevilla Ave. Reina Mercedes s/n 41012 Seville Spain

6. Bioinformatics Wageningen University & Research 6708 PB Wageningen the Netherlands

7. Department of Systems Biology Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma de Madrid 28049 Madrid Spain

Abstract

Summary The Arabidopsis thaliana transcription factor BRANCHED1 (BRC1) plays a pivotal role in the control of shoot branching as it integrates environmental and endogenous signals that influence axillary bud growth. Despite its remarkable activity as a growth inhibitor, the mechanisms by which BRC1 promotes bud dormancy are largely unknown. We determined the genome‐wide BRC1 binding sites in vivo and combined these with transcriptomic data and gene co‐expression analyses to identify bona fide BRC1 direct targets. Next, we integrated multi‐omics data to infer the BRC1 gene regulatory network (GRN) and used graph theory techniques to find network motifs that control the GRN dynamics. We generated an open online tool to interrogate this network. A group of BRC1 target genes encoding transcription factors (BTFs) orchestrate this intricate transcriptional network enriched in abscisic acid‐related components. Promoter::β‐GLUCURONIDASE transgenic lines confirmed that BTFs are expressed in axillary buds. Transient co‐expression assays and studies in planta using mutant lines validated the role of BTFs in modulating the GRN and promoting bud dormancy. This knowledge provides access to the developmental mechanisms that regulate shoot branching and helps identify candidate genes to use as tools to adapt plant architecture and crop production to ever‐changing environmental conditions.

Funder

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Publisher

Wiley

Subject

Plant Science,Physiology

Reference54 articles.

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