Affiliation:
1. Department of Animal Science, Faculty of Agriculture Kasetsart University Bangkok Thailand
2. Food Safety Innovation Establishment Project, Faculty of Agriculture at Kamphaeng Saen Kasetsart University Nakhon Pathom Thailand
3. Faculty of Agricultural Technology, King Mongkut's Institute of Technology Bangkok Thailand
4. Faculty of Agriculture University of Miyazaki Miyazaki Japan
Abstract
AbstractBetong chicken (KU line) is a slow‐growing Thai native chicken used for meat production. The objectives of this study were to identify polymorphisms of the calpain1 (CAPN1) and calpain3 (CAPN3) genes and to investigate their effects on growth, carcass, and meat quality traits in Betong chickens (KU line). A sample of 252 Betong chickens (KU line) was screened for CAPN1 and CAPN3 polymorphisms. The polymorphisms of CAPN1 were detected using gel electrophoresis and DNA sequencing, whereas the polymorphisms of CAPN3 were identified using restriction fragment length polymorphism. Polymorphisms were detected in both CAPN1 (AA, AB, and BB genotypes) and CAPN3 (CC, CT, and TT genotypes). The frequency of the B allele was higher than for the A allele (0.78 and 0.22, respectively) in CAPN1, while the C allelic frequency was higher than for the T allele (0.54 and 0.46, respectively) in CAPN3. The CAPN1 genotype and the combination of the CAPN1 and CAPN3 genotypes could be used as genetic markers for meat lightness. The CAPN3 could be useful for increasing body weight, live weight, and breast meat weight in Betong chickens (KU line).