Affiliation:
1. College of Life Sciences Henan Normal University Xinxiang 453007 China
2. State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China
3. Date Palm Research Institute Shah Abdul Latif University Khairpur Sindh 66020 Pakistan
Abstract
Summary
The sex‐determining‐region (SDR) may offer the best prospects for studying sex‐determining gene, recombination suppression, and chromosome heteromorphism. However, current progress of SDR identification and cloning showed following shortcomings: large near‐isogenic lines need to be constructed, and a relatively large population is needed; the cost of whole‐genome sequencing and assembly is high.
Herein, the X/Y chromosomes of Spinacia oleracea L. subsp. turkestanica were successfully microdissected and assembled using single‐chromosome sequencing.
The assembly length of X and Y chromosome is c. 192.1 and 195.2 Mb, respectively. Three large inversions existed between X and Y chromosome. The SDR size of X and Y chromosome is c. 13.2 and 24.1 Mb, respectively. MSY region and six male‐biased genes were identified. A Y‐chromosome‐specific marker in SDR was constructed and used to verify the chromosome assembly quality at cytological level via fluorescence in situ hybridization. Meanwhile, it was observed that the SDR located on long arm of Y chromosome and near the centromere.
Overall, a technical system was successfully established for rapid cloning the SDR and it is also applicable to rapid assembly of specific chromosome in other plants. Furthermore, this study laid a foundation for studying the molecular mechanism of sex chromosome evolution in spinach.
Funder
National Natural Science Foundation of China
Cited by
2 articles.
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