Sinigrin improves cerebral ischaemia‐reperfusion injury by inhibiting the TLR4 pathway‐mediated oxidative stress

Abstract

AbstractCerebral ischaemia‐reperfusion (CIR) injury occurs in stroke patients after the restoration of cerebral perfusion. Sinigrin, a phytochemical found in cruciferous vegetables, exhibits strong antioxidant activity. This study investigated the role of sinigrin in oxidative stress using a CIR injury model. The effects of sinigrin were studied in middle cerebral artery occlusion (MCAO) rats and oxygen–glucose deprivation/reoxygenation (OGD/R)‐injured SH‐SY5Y cells. Sinigrin treatment improved brain injury and neurological deficits induced by MCAO surgery in rats. Sinigrin inhibited apoptosis in brain tissues and SH‐SY5Y cells following OGD/R induction. Additionally, sinigrin elevated the levels of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH‐Px) while reducing malondialdehyde (MDA) levels. Furthermore, sinigrin inhibited the toll‐like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88) signalling pathway. The anti‐apoptotic and antioxidant activities of sinigrin in OGD/R‐injured SH‐SY5Y cells were reversed by TLR4 overexpression. In conclusion, sinigrin inhibits oxidative stress in CIR injury by suppressing the TLR4/MyD88 signalling pathway.