A classic screening marker does not affect antennal electrophysiology but strongly regulates reproductive behaviours in Bactrocera dorsalis

Author:

Wuyun QiQige1,Zhang Yan1,Yuan Jinxi1,Zhang Jie1,Ren Cong1,Wang Qi1,Yan Shanchun1,Liu Wei2ORCID,Wang Guirong2

Affiliation:

1. Key Laboratory of Sustainable Management of Forest Ecosystem Ministry of Education, Northeast Forestry University Harbin China

2. Institute of Agricultural Genome, Chinese Academy of Agricultural Sciences (Shenzhen), Shenzhen Branch of Lingnan Modern Agricultural Science and Technology Laboratory, Key Laboratory of Agricultural Gene Data Analysis Ministry of Agriculture and Rural Affairs Shenzhen China

Abstract

AbstractThe key phenotype white eye (white) has been used for decades to selectively remove females before release in sterile insect technique programs and as an effective screening marker in genetic engineering. Bactrocera dorsalis is a representative tephritid pest causing damage to more than 150 fruit crops. Yet, the function of white in important biological processes remains unclear in B. dorsalis. In this study, the impacts of the white gene on electrophysiology and reproductive behaviour in B. dorsalis were tested. The results indicated that knocking out Bdwhite disrupted eye pigmentation in adults, consistent with previous reports. Bdwhite did not affect the antennal electrophysiology response to 63 chemical components with various structures. However, reproductive behaviours in both males and females were significantly reduced in Bdwhite−/−. Both pre‐copulatory and copulation behaviours were significantly reduced in Bdwhite−/−, and the effect was male‐specific. Mutant females significantly delayed their oviposition towards γ‐octalactone, and the peak of oviposition behaviour towards orange juice was lost. These results show that Bdwhite might not be an ideal screening marker in functional gene research aiming to identify molecular targets for behaviour‐modifying chemicals. Instead, owing to its strong effect on B. dorsalis sexual behaviours, the downstream genes regulated by Bdwhite or the genes from white‐linked areas could be alternate molecular targets that promote the development of better behavioural modifying chemical‐based pest management techniques.

Funder

Shenzhen Science and Technology Innovation Program

National Key Research and Development Program of China

Publisher

Wiley

Subject

Insect Science,Genetics,Molecular Biology

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