Affiliation:
1. Department of Immunology Molecular and Cell Biology Research Center, School of Medicine, Mazandaran University of Medical Sciences Sari Iran
2. Student Research Committee School of Medicine, Mazandaran University of Medical Sciences Sari Iran
Abstract
AbstractBackgroundCold atmospheric plasma (CAP), is a technology based on non‐thermal ionized gas that is used for cancer therapy in research. We evaluated the effect of CAP on malignant melanoma cancer cell line (B16) in comparison with normal cells (L929).MethodsThe effect of CAP on the cytotoxicity of B16 and L929 cell lines was assayed by the MTT method and inverted microscopy. The induction of apoptosis in cells was evaluated using a fluorescence microscope. FTIR monitored the CAP effect in biomacromolecules changes in these cell lines. QPCR assayed gene expression of BAX, BCL‐2, and Caspase‐3 (CASP‐3).ResultsThe results of the MTT test showed CAP has a cytotoxic effect on the B16 cancer cell line more than L929 normal cells (p < 0.0001). The results of invert and fluorescence microscopy showed CAP‐induced apoptotic morphology on cancerous cells. FTIR spectroscopy indicated CAP changes biomacromolecules structure. Evaluation of gene expression showed CAP increased BAX and CASP‐3 gene expression. Also, it decreased BCL‐2 gene expression.ConclusionsTaken together, CAP may change biomacromolecule structures involved in apoptosis pathways, decrease proliferation and induce apoptosis in cancer cells.