Mutants of Ca2+-regulated Photoprotein Obelin for Site-specific Conjugation

Author:

Krasitskaya Vasilisa V.1,Burakova Ludmila P.1,Komarova Anastasia A.2,Bashmakova Eugenia E.12,Frank Ludmila A.12

Affiliation:

1. Institute of Biophysics SB RAS; Federal Research Center “Krasnoyarsk Science Center SB RAS”; Krasnoyarsk Russia

2. Siberian Federal University; Krasnoyarsk Russia

Funder

Russian Foundation for Basic Research

Russian Academy of Sciences

Publisher

Wiley

Subject

Physical and Theoretical Chemistry,General Medicine,Biochemistry

Reference18 articles.

1. Structure of the Ca2+-regulated photoprotein obelin at 1.7 Å resolution determined directly from its sulfur substructure;Liu;Protein Sci.,2000

2. Semisynthetic photoprotein reporters for tracking fast Ca2+ transients;Malikova;Photochem. Photobiol. Sci.,2015

3. Application of enzyme bioluminescence for medical diagnostics;Frank;Adv. Biochem. Eng. Biotechnol.,2014

4. Bioluminescent immunoassay of thyrotropin and thyroxine using obelin as a label;Frank;Anal. Biochem.,2004

5. Conjugates of the Ca2+-regulated photoprotein obelin with immunoglobulins: Synthesis and use as labels in bioluminescent immunoassay;Frank;Bioorg. Khim.,2004

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