Standardized methods to generate mock (spiked) clinical specimens by spiking blood or plasma with cultured pathogens

Author:

Dong M.1,Fisher C.1,Añez G.1,Rios M.1,Nakhasi H.L.1,Hobson J.P.2,Beanan M.3,Hockman D.4,Grigorenko E.4,Duncan R.1ORCID

Affiliation:

1. Laboratory of Emerging Pathogens; Division of Emerging and Transfusion Transmitted Diseases; Center for Biologics Evaluation and Research (CBER); Food and Drug Administration (FDA); Silver Spring MD USA

2. Office of In Vitro Diagnostics and Radiological Health (OIR); Division of Microbiology Devices; Center for Devices and Radiological Health (CDRH); FDA; Silver Spring MD USA

3. Office of Biodefense, Research Resources, and Translational Research; Division of Microbiology and Infectious Diseases (DMID); National Institute of Allergy and Infectious Diseases (NIAID); Bethesda MD USA

4. Diatherix Laboratory; Huntsville AL USA

Funder

National Institute of Allergy and Infectious Diseases

Interagency Agreement

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Reference18 articles.

1. Rapid image-based cytometry for comparison of fluorescent viability staining methods;Chan;J Fluoresc,2012

2. Expression of calreticulin P-domain results in impairment of secretory pathway in Leishmania donovani and reduced parasite survival in macrophages;Debrabant;Int J Parasitol,2002

3. Generation of Leishmania donovani axenic amastigotes: their growth and biological characteristics;Debrabant;Int J Parasitol,2004

4. [Isolation of Yersinia pestis and Yersinia pseudotuberculosis by amplifying 16S rRNA and its genes];Drygin Iu;Mol Biol (Mosk),1995

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