Extracellular vesicle features are associated with COVID‐19 severity

Author:

Caponnetto Federica1,De Martino Maria1,Stefanizzi Daniele1,Del Sal Riccardo1,Manini Ivana1,Kharrat Feras1,D'Aurizio Federica2,Fabris Martina12,Visentini Daniela2,Poz Donatella2,Sozio Emanuela2,Tascini Carlo12,Cesselli Daniela12,Isola Miriam1,Beltrami Antonio Paolo12ORCID,Curcio Francesco12

Affiliation:

1. Department of Medicine University of Udine Udine Italy

2. Azienda Sanitaria Universitaria Friuli Centrale Udine Italy

Abstract

AbstractCOVID‐19 is heterogeneous; therefore, it is crucial to identify early biomarkers for adverse outcomes. Extracellular vesicles (EV) are involved in the pathophysiology of COVID‐19 and have both negative and positive effects. The objective of this study was to identify the potential role of EV in the prognostic stratification of COVID‐19 patients. A total of 146 patients with severe or critical COVID‐19 were enrolled. Demographic and comorbidity characteristics were collected, together with routine haematology, blood chemistry and lymphocyte subpopulation data. Flow cytometric characterization of the dimensional and antigenic properties of COVID‐19 patients' plasma EVs was conducted. Elastic net logistic regression with cross‐validation was employed to identify the best model for classifying critically ill patients. Features of smaller EVs (i.e. the fraction of EVs smaller than 200 nm expressing either cluster of differentiation [CD] 31, CD 140b or CD 42b), albuminemia and the percentage of monocytes expressing human leukocyte antigen DR (HLA‐DR) were associated with a better outcome. Conversely, the proportion of larger EVs expressing N‐cadherin, CD 34, CD 56, CD31 or CD 45, interleukin 6, red cell width distribution (RDW), N‐terminal pro‐brain natriuretic peptide (NT‐proBNP), age, procalcitonin, Charlson Comorbidity Index and pro‐adrenomedullin were associated with disease severity. Therefore, the simultaneous assessment of EV dimensions and their antigenic properties complements laboratory workup and helps in patient stratification.

Publisher

Wiley

Subject

Cell Biology,Molecular Medicine

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