A mini‐Tn5‐derived transposon with reportable and selectable markers enables rapid generation and screening of insertional mutants in Gram‐negative bacteria
Author:
Affiliation:
1. Department of Plant Pathology University of Minnesota St. Paul MN USA
2. Department of Agricultural and Environmental Sciences Tennessee State University Nashville TN USA
Funder
National Institute of Food and Agriculture
Publisher
Wiley
Subject
Applied Microbiology and Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1111/lam.13423
Reference26 articles.
1. Circular permutation profiling by deep sequencing libraries created using transposon mutagenesis
2. Approaches to querying bacterial genomes with transposon-insertion sequencing
3. Assaying Promoter Activity Using LacZ and GFP as Reporters
4. Electrotransformation of Pseudomonas
5. Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes
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1. Tn5 Transposon-based Mutagenesis for Engineering Phage-resistant Strains of Escherichia coli BL21 (DE3);Journal of Microbiology;2023-05
2. Phage resistance of Salmonella enterica obtained by transposon Tn5‐mediated SefR gene silent mutation;Journal of Basic Microbiology;2023-04-09
3. Homologous recombination risk in baculovirus expression vector system;Virus Research;2022-11
4. Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria;Current Opinion in Biotechnology;2022-10
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