Photodynamic inactivation of different pathogenic bacteria on human skin using a novel photosensitizer hydrogel

Author:

Eckl Daniel Bernhard12,Hoffmann Anja Karen1,Landgraf Nicole1,Kalb Larissa2,Bäßler Pauline2,Wallner Susanne2,Eichner Anja3,Huber Harald1,Hackbarth Steffen4,Bäumler Wolfgang2ORCID

Affiliation:

1. Institute for Microbiology and Archaea Centre University of Regensburg Regensburg Germany

2. Department of Dermatology University Hospital Regensburg Regensburg Germany

3. Department of Infection Control and Infectious Diseases University Hospital Regensburg Germany

4. Photobiophysics, Institute of Physics Humboldt University of Berlin Berlin Germany

Abstract

AbstractBackgroundThe colonization of skin with pathogenic, partially antibiotic‐resistant bacteria is frequently a severe problem in dermatological therapies. For instance, skin colonization with Staphylococcus aureus is even a disease‐promoting factor in atopic dermatitis. The photodynamic inactivation (PDI) of bacteria could be a new antibacterial procedure. Upon irradiation with visible light, a special photosensitizer exclusively generates singlet oxygen. This reactive oxygen species kills bacteria via oxidation independent of species or strain and their antibiotic resistance profile causing no bacterial resistance on its part.ObjectiveTo investigate the antibacterial potential of a photosensitizer, formulated in a new hydrogel, on human skin ex vivo.MethodsThe photochemical stability of the photosensitizer and its ability to generate singlet oxygen in the hydrogel was studied. Antimicrobial efficacy of this hydrogel was tested step by step, firstly on inanimate surfaces and then on human skin ex vivo against S. aureus and Pseudomonas aeruginosa using standard colony counting. NBTC staining and TUNEL assays were performed on skin biopsies to investigate potential necrosis and apoptosis effects in skin cells possibly caused by PDI.ResultsNone of the hydrogel components affected the photochemical stability and the life time of singlet oxygen. On inanimate surfaces as well as on the human skin, the number of viable bacteria was reduced by up to 4.8 log10 being more effective than most other antibacterial topical agents. Histology and assays showed that PDI against bacteria on the skin surface caused no harmful effects on the underlying skin cells.ConclusionPhotodynamic inactivation hydrogel proved to be effective for decolonization of human skin including the potential to act against superficial skin infections. Being a water‐based formulation, the hydrogel should be also suitable for the mucosa. The results of the present ex vivo study form a good basis for conducting clinical studies in vivo.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Infectious Diseases,Dermatology

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