Disposition of Heptabarbitone in the Rat: Identification of a New Metabolite by Tandem Mass Spectrometry-Reference-Cited by-同舟云学术

Disposition of Heptabarbitone in the Rat: Identification of a New Metabolite by Tandem Mass Spectrometry

Published:1992-11 Issue:11 Volume:44 Page:907-910
ISSN:2042-7158
Container-title:Journal of Pharmacy and Pharmacology
language:en
Short-container-title:

Author:

Stijnen A M¹,Heeremans C E M²,Van Bezooijen C F A³,Niessen W M A²,Danhof M¹

Affiliation:

1. Center for Bio-Pharmaceutical Sciences, Division of Pharmacology, University of Leiden, PO Box 9503, 2300 RA Leiden, The Netherlands

2. Center for Bio-Pharmaceutical Sciences, Division of Analytical Chemistry, University of Leiden, PO Box 9502, 2300 RA Leiden, The Netherlands

3. TNO institute for Ageing and Vascular Research, PO Box 2215, 2301 CE, Leiden, The Netherlands

Abstract

Abstract The purpose of this study was to elucidate the structure of a metabolite of heptabarbitone, the occurrence of which was reported previously in a number of pharmacokinetic and pharmacodynamic modelling studies. By application of thermospray liquid chromatography (tandem) mass spectrometry, the identity of the metabolite was proposed to be 5-ethyl-5-(1′,[3′ or 6′]-cycloheptadienyl)-barbituric acid. By measuring the ratios between the areas under the concentration time curves of the metabolite and heptabarbitone after administration of three different intravenous dosages of heptabarbitone, it was shown that the exposure to the metabolite is directly correlated with the exposure of heptabarbitone.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Link

http://academic.oup.com/jpp/article-pdf/44/11/907/37241890/j.2042-7158.1992.tb03234.x.pdf

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