Neopterin, 7,8‐dihydroneopterin, total neopterin levels and their ratio in periodontitis: New dilemma

Author:

Turkmen Emrah1,Sayedyousef Hadi12,Ucar Dorukhan12ORCID,Yılmaz Melis1ORCID,Akdoğan Nihan3ORCID,Balci Nur1,Kurgan Şivge3,Toygar Hilal1,Serdar Muhittin A.4

Affiliation:

1. Faculty of Dentistry, Department of Periodontology Istanbul Medipol University Istanbul Turkey

2. Department of Periodontology, Institute of Health Sciences Istanbul Medipol University Istanbul Turkey

3. Department of Periodontology Faculty of Dentistry Ankara University Ankara Turkey

4. Department of Medical Biochemistry, School of Medicine Acıbadem University Ankara Turkey

Abstract

AbstractObjectiveDetermine the saliva and serum levels of neopterin (NP) and 7,8‐dihydroneopterin (7,8NP) in periodontitis patients and to reveal the relationship of these data with clinical periodontal parameters.Materials and MethodsTwenty‐three patients with stage III/grade B periodontitis and 23 periodontally healthy individuals were included. Clinical periodontal measurements were recorded (plaque index, pocket depth, clinical attachment loss & bleeding on probing). Saliva and serum levels of NP and 7,8NP were analyzed by high‐performance liquid chromatography.ResultsSaliva NP, 7,8NP and Total Neopterin (TNP) levels were significantly elevated in the periodontitis than the control group (p < 0.001).ROC analyses of saliva NP, 7,8NP and TNP yielded areas under the curves of 0.873–0.938 for discriminating periodontitis from health, and saliva TNP was found the most accurate biomarker (AUC = 0.938).There was no significant difference among the periodontitis and control groups for saliva TNP/NP and TNP/7,8NP ratios and serum NP, 7,8NP and TNP levels (p > 0.05).ConclusionIncreased saliva TNP, NP and 7,8NP levels in periodontitis may suggest these biomarkers are regulating immune activation and oxidative stress mechanism in periodontal inflammation. Additionally, together with these results, equivalence of the TNP/NP ratio in intergroups may suggest that the effects of immune activation and oxidative stress mechanisms are equal in the periodontitis.

Publisher

Wiley

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