Affiliation:
1. College of Animal Science and Technology, Hebei Technology Innovation Center of Cattle and Sheep Embryo Hebei Agricultural University Baoding China
2. Institute of Xinjiang Yili Animal Science Xinjiang China
3. Dental Stem cell Bank and Research Center Savaid Stomatology School of Hangzhou Medical College Hangzhou China
Abstract
AbstractHuman menopausal gonadotrophin (hMG) has been reported to produce a comparable superovulatory response to that of follicle stimulating hormone (FSH). Furthermore, hMG has a long half‐life as compared with FSH. The present study was designed to compare hMG administered once daily and FSH administered twice daily over a 4 ‐ day period on superovulatory response of Suffolk ewes. During the mid‐luteal phase, twenty‐four Suffolk donor ewes received intravaginal sponges at day 0 for 12 days. The superovulatory regimens in the Control group (n = 12) and the Treatment group (n = 12) consisted of eight injections of FSH given at twice daily and four injections of hMG given at once daily, respectively. At day 13, the donor ewes were subjected to laparoscopic insemination. Embryos were recovered, classified, and transferred to recipient ewes at day 19. Pregnancy status was determined by ultrasound examination 40 days after transfer. Lambing rate was calculated after all the ewes had delivered. No significant differences were observed between the two groups in terms of the structures recovered, transferable embryos, degenerated embryos, unfertilized oocytes, pregnancy rate and lambing rate. The results showed that once daily injection of hMG can produce a comparable superovulatory response and embryo transfer outcomes to those obtained by twice daily injection of FSH over a 4 ‐ day period. It is feasible that hMG is used to replace FSH and reduce the number of injection treatments in ovine superovulatory regimens.
Funder
Key Research and Development Program of Hebei Province
Natural Science Foundation of Hebei Province
Subject
Endocrinology,Animal Science and Zoology,Biotechnology