Topography of the respiratory, oral, and guttural pouch bacterial and fungal microbiotas in horses

Author:

Bond Stephanie12ORCID,McMullen Christopher13ORCID,Timsit Edouard14ORCID,Léguillette Renaud1ORCID

Affiliation:

1. Faculty of Veterinary Medicine University of Calgary Calgary Alberta Canada

2. School of Veterinary Science, Faculty of Science University of Queensland Gatton Australia

3. Feedlot Health Management Services, Inc Okotoks Alberta Canada

4. I&D Pharma Departement Ceva Santé Animale Libourne France

Abstract

AbstractBackgroundThe lower respiratory tract microbiota of the horse is different in states of health and disease, but the bacterial and fungal composition of the healthy respiratory tract of the horse has not been studied in detail.HypothesisThe respiratory tract environment contains distinct niche microbiotas, which decrease in species richness at more distal sampling locations.ObjectiveCharacterize the bacterial and fungal microbiotas along the upper and lower respiratory tract of the horse.AnimalsHealthy Argentinian Thoroughbred horses (n = 11) from the same client‐owned herd.MethodsProspective cross‐sectional study. Eleven upper and lower respiratory tract anatomical locations (bilateral nasal, bilateral deep nasal, nasopharynx, floor of mouth, oropharynx, arytenoids, proximal and distal trachea, guttural pouch) were sampled using a combination of swabs, protected specimen brushes, and saline washes. Total DNA was extracted from each sample and negative control, and the 16S rRNA gene (V4) and ITS2 region were sequenced. Community composition, alpha‐diversity, and beta‐diversity were compared among sampling locations.ResultsFungal species richness and diversity were highest in the nostrils. More spatial heterogeneity was found in bacterial composition than in fungal communities. The pharyngeal microbiota was most similar to the distal tracheal bacterial and fungal microbiota in healthy horses and therefore may serve as the primary source of bacteria and fungi to the lower respiratory tract.Conclusions and Clinical ImportanceThe pharynx is an important location that should be targeted in respiratory microbiota research in horses. Future studies that investigate whether biomarkers of respiratory disease can be reliably detected in nasopharyngeal swab samples are warranted.

Publisher

Wiley

Subject

General Veterinary

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