Affiliation:
1. Sydney School of Veterinary Science, Faculty of Science The University of Sydney Sydney New South Wales 2006 Australia
2. McIvor Road Veterinary Centre Bendigo Victoria Australia
3. Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Centre University of New South Wales Sydney New South Wales Australia
Abstract
AbstractBackgroundChronic enteropathy (CE) is common in dogs and can occur with multiple etiologies including food‐responsive enteropathy (FRE) and idiopathic inflammatory bowel disease (IBD).Hypothesis/ObjectiveTo study the protein profile and pathway differences among dogs with FRE, IBD, and healthy controls using serum proteome analysis.AnimalsNine CE dogs with signs of gastrointestinal disease and histologically confirmed chronic inflammatory enteropathy and 16 healthy controls.MethodsA cross‐sectional study with cases recruited from 2 veterinary hospitals between May 2019 and November 2020 was performed. Serum samples were analyzed using mass spectrometry‐based proteomic techniques.ResultsProteomic profiles showed marked variation in relative protein abundances. Forty‐five proteins were significantly (P ≤ .01) differentially expressed among the dogs with CE and controls with ≥2‐fold change in abundance. The fold change of dogs with IBD normalized to controls was more pronounced for the majority of proteins than that seen in the dogs with FRE normalized to control dogs. Proteins involving reactive oxygen species, cytokine activation, acute phase response signaling, and lipid metabolism were altered in dogs with CE.Conclusions and Clinical ImportanceCytokine alterations, acute phase response signaling, and lipid metabolism are likely involved in pathogenesis of CE. Although there are insufficient current data to justify the use of proteomic biomarkers for assessment of CE in dogs, our study identifies potential candidates.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献