Cardioprotective effect of Salvianolic acid B on acute myocardial infarction by promoting autophagy and neovascularization and inhibiting apoptosis

Author:

Lin Chao1,Liu Zhaoguo1,Lu Ying1,Yao Yuan1,Zhang Yayun1,Ma Zhi1,Kuai Meiyu1,Sun Xin1,Sun Shuaijun1,Jing Yi1,Yu Lizhen1,Li Yu2,Zhang Qichun13,Bian Huimin13

Affiliation:

1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China

2. Department of Preclinical Medicine, Nanjing University of Chinese Medicine, Nanjing, China

3. Jiangsu Key Laboratory for Pharmacolgy and Safety Evaluation of Chinese Materia Medica, Nanjing, China

Abstract

Abstract Objectives The aim of this study was to investigate the cardioprotective effect of salvianolic acid B (Sal B) on acute myocardial infarction (AMI) in rats and its potential mechanisms. Methods The AMI model was established in rats to study the effect of Sal B on AMI. Haematoxylin–eosin (HE) staining was used to evaluate the pathological change in AMI rats. Immunofluorescence and TUNEL staining were used to detect autophagy and apoptosis of myocardial cells in hearts of AMI rats, respectively. Protein expression of apoptosis-related, autophagy-related and angiogenesis-related proteins were examined by Western blot. Key findings Sal B attenuated myocardial infarction significantly compared with that of the model group. Rats administered with Sal B showed higher inhibition rate of infarction and lower infarct size than those of the model group. Moreover, Sal B decreased the serum levels of creatine kinase, lactate dehydrogenase and malondialdehyde, while increased such level of superoxide dismutase significantly compared with those of the model group. Sal B inhibited the expression of Bax, cleaved caspase-9 and cleaved PARP, while promoted the expression of Bcl-2, LC3-II, Beclin1 and VEGF. Conclusions Sal B has cardioprotective effect on AMI and Sal B may be a promising candidate for AMI treatment.

Funder

National Natural Science Foundation of China

Priority Academic Program Development of Jiangsu Higher Education Institutions

Project Management Bureau in Jiangsu Province

National Science and Technology Major Project of the Ministry of Science and Technology of China-Significant New Drugs Creation

Postgraduate Research & Innovation Project of Jiangsu Province

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

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