Affiliation:
1. Department of Periodontology School of Dentistry Pontificia Universidad Católica Madre y Maestra Santo Domingo Dominican Republic
2. Oral Microbiology and Biomaterials Laboratory Department of Biomedical Surgical and Dental Sciences, University of Milan Milan Italy
3. Bioscience Research Center College of Dentistry University of Tennessee Health Science Center, Memphis TN and The Forsyth Institute Cambridge Massachusetts USA
Abstract
AbstractAerosols produced by dental handpieces represent a permanent risk of disease transmission in the dental environment. The current study evaluated the effects of natural ventilation (open windows) on Streptococcus mutans airborne contamination by dental handpieces in simulated clinical conditions. A dental phantom was placed on a dental chair at a standard university dental clinic operatory. An S. mutans suspension was infused into the phantom's mouth while an operator performed standardized dental procedures using low (contra‐angle) and high speed (turbine) dental handpieces or an ultrasonic scaler, with windows open or closed. Selective medium Petri dishes were placed in 18 sites of the operatory environment to evaluate contamination topographically. Sites were clustered as: wall, floor, ceiling, dental chair, and cabinet. Contamination was expressed as log10CFU/cm2. A linear mixed model analysis was used, nesting the sites within each ventilation and handpiece combination. Open windows significantly reduced contamination. The high‐speed handpiece provided the highest contamination, followed by the ultrasonic scaler and the low‐speed handpiece. Contamination values were much smaller at the ceiling, and much larger at the chair. Opening windows produced more homogeneous contamination of the operatory compared to closed windows. Natural ventilation during dental procedures decreases contamination and affects its topographical distribution.