Affiliation:
1. College of Horticulture Gansu Agricultural University Lanzhou China
2. Vegetable Institute Gansu Academy of Agricultural Sciences Lanzhou China
Abstract
AbstractCytoplasmic male sterility (CMS) is a very important factor to produce hybrid seeds, and the restoration of fertility involves the expression of many fertility‐related genes. Our previous study showed that the expression of CaPIPLC5 was significantly up‐regulated in pepper restorer accessions and minimally expressed in sterile accessions, speculating that CaPIPLC5 is related to the restoration of fertility. In this study, we further validated the function of CaPIPLC5 in the restoration of fertility. The results showed that CaPIPLC5 was specifically expressed in the anthers of the restorer accessions with the subcellular localization in the cytoplasm. Furthermore, the expression of CaPIPLC5 was significantly higher in restorer lines and restorer combinations than that in CMS lines and their maintainer lines. Silencing CaPIPLC5 led to the number of pollen decreased, pollen grains wrinkled, and the ratio of pollen germination reduced. In addition, the joint analysis of Yeast One‐Hybrid (Y1H) and Dual‐Luciferase (dual‐LUC) assays suggested that transcription factors such as CaARF5, CabZIP24 and CaMYB‐like1, interacted with the promoter regions of CaPIPLC5, which regulated the expression of CaPIPLC5. The present results provide new insights into the study of CaPIPLC5 involved in the restoration of fertility in pepper.