Abstract
Lipopolysaccharide (LPS) was isolated from an avian strain of Escherichia coli O18 by extraction of the cells with NaCl followed by treatment with phenol–water, methanol precipitation, and gel permeation chromatography. The LPS consisted of carbohydrate, lipid, and a small amount of protein and phosphate. Further analysis showed the presence of glucose, galactose, rhamnose, glucosamine, N-acetylglucosamine, heptose, 2-keto-3-deoxyoctonate (KDO), and ethanolamine. The composition of the LPS is qualitatively similar to that found for LPS obtained from various other bacteria.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology