A FERMENTATION TUBE AND MEDIUM FOR FASTIDIOUS ANAEROBES

Abstract

A modified fermentation tube was devised by introducing a glass rod in the small inverted Durham tube to keep its mouth away from the bottom of the outer main tube, thus providing the maximum opportunity for gas collection. A medium containing autoclavable growth factors was developed, which, when added to the main tubes with a few particles of cooked meat, allowed growth of fastidious anaerobes. Each ingredient was shown to be free of any fermentable substance that might lead to gas formation. The fermentation tubes were incubated in an anaerobic jar.