Physiological analysis of the lymphatic system in the eastern painted turtle (Chrysemys picta picta)

Abstract

Examination into the anuran lymphatic system has led to a comprehensive understanding of lymphatics, including the importance of synchrony in fluid-balance maintenance. However, little research has been conducted on the lymphatics of turtles and other reptilian vertebrates. Using pressure-peak recordings created through cannulation of both lymph hearts of the eastern painted turtle, Chrysemys picta picta (Schneider, 1783), the lymph heart contraction rate was verified and the interbeat interval patterns were examined using Poincaré plots. The lymph heart beating rate was determined to be 38.2 beats·min–1with a mean pulse pressure of 2.40 ± 1.44 mm Hg (1 mm Hg at 0 °C = 133.3224 Pa). Poincaré plots are useful in displaying nonlinear sequential data and are often given descriptive names related to the overall pattern. The Poincaré plot resembled a garden hose nozzle spray, indicating a large variability in interbeat time intervals with periods of multiple-beat patterns. The degree of bilateral lymph heart synchrony was determined in the turtle using the mean time difference between right and left lymph heart systoles. Results show that chelonian lymph hearts do in fact beat in synchrony, with over 50% of contractions occurring within 100 ms of each other. This indicates shared neuronal control and may suggest an energetic advantage to fluid homeostasis.