Negative chemotaxis inCytophaga johnsonae

Abstract

Chemotaxis, both positive and negative, has been extensively studied in flagellated bacteria, such as Escherichia coli and Salmonella typhimurium, but not in gliding bacteria. The rapidly motile gliding bacterium Cytophaga johnsonae has been seen to be repelled by H2O2, OCl−, and N-chlorotaurine, as well as by low pH. Its response to H2O2was eliminated by catalase. Nalidixic acid at 200 μM, which inhibits the growth but not the motility of C. johnsonae, did not interfere with its negative chemotactic response to H2O2, whereas sodium phosphate at 10 mM, which inhibits motility, did so. Cytophaga johnsonae was not repelled by taurine, n-octanol, phenol, L-valine, or high pH. Chemotaxis can be conveniently studied in gliding bacteria such as C. johnsonae.Key words: gliding bacteria, Cytophaga johnsonae, negative chemotaxis, hydrogen peroxide, N-chlorotaurine.