The isolation of estrone sulfate and estradiol-17β sulfate from stallion testes

Abstract

A method is described for the extraction and purification of estrogen sulfates from testicular tissues. By use of this method estrone 3-sulfate and estradiol-17β 3-sulfate were isolated from stallion testes and identified by infrared spectroscopy. Further characterization was effected by the use of estrone-4-14C and estrone-6,7-3H 3-sulfate as standards for chromatographic comparison. Preliminary results indicated that the amounts of the estrogens present as sulfates were greatly in excess of the amounts present as free steroid. Estradiol-17α could not be detected.