REQUIREMENTS FOR AND RATES OF FORMATION OF EXTRACELLULAR PSEUDOURIDINE BY AGROBACTERIUM TUMEFAGIENS

Abstract

The minimal requirements for a synthetic medium capable of supporting adequate growth of Agrobacterium tumefaciens were found to be D-glucose, urea, phosphate, and magnesium salt. D-Ribose, D-fructose, and D-xylose could be used to replace D-glucose.When the organism was grown on the above medium, the addition of any one of the pyrimidines or pyrimidine ribosides, uracil, cytidine, uridine, or orotic acid resulted in the extracellular accumulation of pseudouridine. From the data presented it is clear that conversion to uracil took place in all cultures before pseudouridine was formed.