The sites of action of praziquantel in a smooth muscle of Lymnaea stagnalis

Abstract

Praziquantel (1.6 × 10−6 M), a new anthelmintic drug, was shown to produce a sustained contracture in the penis retractor muscle of the freshwater snail Lymnaea stagnalis. Calcium-free saline greatly reduced the magnitude and duration of the praziquantel contractures if the preparation had been previously exposed to praziquantel. The praziquantel contracture in zero-calcium saline was abolished if the preparation was pretreated with successive applications of 2 × 10−2 M caffeine. Lanthanum (1.0 mM) in zero-calcium saline prevented the contractures normally produced by 30 mM potassium saline, but did not prevent the praziquantel contracture. In normal saline, however, 1.0 mM lanthanum greatly reduced the magnitude of a praziquantel-induced contracture. We conclude that praziquantel increases sarcolemmal calcium permeability and releases calcium from intracellular stores in Lymnaea stagnalis smooth muscle.