A thin-layer chromatographic study of the phenolics of Triticale and its parental species

Abstract

The phenolics of young leaves from a hexaploid and three octaploid Triticale lines, and their rye and wheat parents were investigated by two dimensional thin-layer chromatography. Matching coefficients, coefficients of similarity, biochemical distances, and correlation coefficients were calculated to determine the degree of similarity of phenolics between the different groups. Chromatographic patterns and the calculated similarity measures showed a close relationship of phenolics between Triticale and its wheat parent. These patterns were even more closely related if the wheat parent was hexaploid. Two wheat varieties (Prelude and Kharkov) had identical chromatographic patterns while the variety Chinese Spring showed a reduced number of spots. The pattern of Stewart, a durum wheat variety, was intermediate between Chinese Spring and Prelude or Kharkov. Generally, the number of flavonoid compounds increased with the level of ploidy but did not increase proportionally with the number of genomes.