Vaccine adjuvant ginsenoside Rg1 enhances immune responses against hepatitis B surface antigen in mice-Reference-Cited by-同舟云学术

Vaccine adjuvant ginsenoside Rg1 enhances immune responses against hepatitis B surface antigen in mice

Published:2016-06 Issue:6 Volume:94 Page:676-681
ISSN:0008-4212
Container-title:Canadian Journal of Physiology and Pharmacology
language:en
Short-container-title:Can. J. Physiol. Pharmacol.

Author:

Yuan Ding¹²,Yuan Qin¹,Cui Qianqian¹,Liu Chaoqi¹,Zhou Zhiyong¹,Zhao Haixia¹,Dun Yaoyan¹,Wang Ting¹,Zhang Changcheng¹

Affiliation:

1. College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.

2. Renhe Hospital, The Second College of Clinical Medical Science, Three Gorges University, Yichang, Hubei 443001, China.

Abstract

The adjuvant effect of ginsenoside Rg1 on immune responses against hepatitis B surface antigen (HBsAg) in mice was investigated. Female BALB/c mice were subcutaneously injected with saline or HBsAg antigen with or without Rg1 on days 7 and 21. Samples were collected 2 weeks after the boosting for the detection of anti-HBsAg immunoglobulin G (IgG) isotypes in sera and gamma interferon (IFN-γ) and interleukin-4 (IL-4) produced in splenocytes. The innate and adaptive immune responses were measured in mice immunized as described above. The results showed that ginsenoside Rg1 had adjuvant properties in stimulating IgG, splenocyte proliferation, and mRNA expression of cytokines IFN-γ and IL-4, as well as the expression of cell surface marker TLR4 in the HBsAg-immunized mice. These results indicate that Rg1 enhances both Th1 (IgG2b and IFN-γ) and Th2 (IgG1 and IL-4) responses. In addition, the TLR4 signaling pathway is involved in the adjuvant activities of ginsenoside Rg1.

Publisher

Canadian Science Publishing

Subject

Physiology (medical),Pharmacology,General Medicine,Physiology

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/cjpp-2015-0528

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