Electrophoresis of dyed polysaccharides on cellulose acetate

Abstract

Polysaccharides have been shown to react with Procion dyes to give colored products. The degree of substitution varies with the dye to polysaccharide ratio used in the reaction and with the structure of the polysaccharide. The dyed polysaccharides give visible bands on gel filtration and on electrophoresis on cellulose acetate strips. Separations of mixtures of colored polysaccharides by the latter method are complete within 5 min and match the results obtained with the undyed polysaccharides by free-boundary, Tiselius electrophoresis.