On the purification of a fatty acid synthetase complex from an insect
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Published:1976-08-01
Issue:8
Volume:54
Page:1397-1399
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ISSN:0008-4301
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Container-title:Canadian Journal of Zoology
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language:en
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Short-container-title:Can. J. Zool.
Author:
Thompson S. N.,Barlow J. S.
Abstract
The fatty acid synthetase complex of the blowfly, Lucilia sericata, catalyzing the de novo synthesis of fatty acids from acetyl- and malonyl-CoA (EC 2.3.1.9 and EC 2.3.1.39 respectively) and requiring NADPH, was isolated from whole-insect homogenates by ultracentrifugation. Purification was carried out by salt fractionation, anion exchange column chromatography on ECTEOLA (epichlorohydrin triethanolamine) cellulose, and gel filtration column chromatography on Sephadex®.
Publisher
Canadian Science Publishing
Subject
Animal Science and Zoology,Ecology, Evolution, Behavior and Systematics
Cited by
1 articles.
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1. Lipid Metabolism;Biochemistry;1985