Differentiation of pathogenic, gram-negative, bacterial genera in the presence of β-2-thienylalanine

Author:

Brown Kurt J.,Lines David R.

Abstract

Described is a fast screening assay for aid in the differentiation of bacterial genera. Salmonella is separated from Escherichia and Shigella, as a generic group on the basis of an inhibition assay incorporating β-2-thienylalanine, and using a defined medium. The assay is also usable for confirmation of taxonomic differences between genera.The inhibition reaction, readable as an 'all-or-none' phenomenon, follows a dose-response relationship for cultures affected by β-2-thienylalanine, and is unaffected by the growth phase of the culture.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

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