Cryptic lineages and hybridization in freshwater mussels of the genus Pyganodon (Unionidae) in northeastern North America

Abstract

The distribution of freshwater mussels Pyganodon Crosse and P. Fischer, 1894 traditionally inferred from morphological characters was validated by a genetic characterization of the genus within the Quebec peninsula. Individuals were identified by comparing the sequences from the female mitochondrial genome (COI and 16S) with those of reference individuals, while hybridization was assessed with male mitochondrial (COI and COII) and nuclear genomes (ITS1 and ITS2). The results confirmed most of the previous morphological identifications but revealed unexpected results. Both male and female mitochondrial genomes support the distinction between Pyganodon fragilis (Lamarck, 1819) and Pyganodon cataracta (Say, 1817). However, only one lineage of Pyganodon grandis (Say, 1829), instead of the two expected, was detected in the sampled area. The genetic survey also revealed the presence of two unidentified Pyganodon lineages, previously unreported within the Quebec peninsula. These extremely rare lineages harbour the signature of ancestral hybridizations. Finally, recent divergence and hybridizations make shell characters only partially efficient in discriminating Pyganodon lineages.