Molecular phylogeny of Ustilago and Sporisorium species (Basidiomycota, Ustilaginales) based on internal transcribed spacer (ITS) sequences

Abstract

DNA sequence data from the internal transcribed spacer (ITS) region of the nuclear rDNA genes were used to determine a phylogenetic relationship between the graminicolous smut genera Ustilago and Sporisorium (Ustilaginales). Fifty-three members of both genera were analysed together with three related outgroup genera. Neighbor-joining and Bayesian inferences of phylogeny indicate the monophyly of a bipartite genus Sporisorium and the monophyly of a core Ustilago clade. Both methods confirm the recently published nomenclatural change of the cane smut Ustilago scitaminea to Sporisorium scitamineum and indicate a putative connection between Ustilago maydis and Sporisorium. Overall, the three clades resolved in our analyses are only weakly supported by morphological characters. Still, their preferences to parasitize certain subfamilies of Poaceae could be used to corroborate our results: all members of both Sporisorium groups occur exclusively on the grass subfamily Panicoideae. The core Ustilago group mainly infects the subfamilies Pooideae or Chloridoideae.Key words: basidiomycete systematics, ITS, molecular phylogeny, Bayesian analysis, Ustilaginomycetes, smut fungi.