Radiation induced peroxidation of polyunsaturated fatty acids: Recent results on formation of hydroperoxides

Abstract

Aqueous solutions of linoleic acid were irradiated in air with γ-rays of137Cs. High pressure liquid chromatography (HPLC) was been used to separate and measure the production of hydroperoxides. The results obtained after reverse phase chromatography, associated with a microperoxydase for hydroperoxide detection, indicate the presence of two different hydroperoxides. One type of hydroperoxide was the major product obtained when the initial linoleic concentrations were below the critical micellar concentration (2 mM), and the second type was produced when the concentrations were above 2 mM. A further separation carried out on the second hydroperoxide by direct phase HPLC showed that it contains three compounds, mainly HPODE 9 and 13.Key words: linoleic acid, peroxidation, hydroperoxide, direct phase HPLC, reverse phase HPLC.