CHANGES IN TISSUE HISTAMINE OF GUINEA PIGS AFTER PRETREATMENT WITH HISTAMINE-RELEASING AGENTS AND ACETYLSALICYLIC ACID

Abstract

Guinea pigs were treated with compound 48/80 and cortisone for 14 days. Seven days after the last dose the animals were killed and their organs extracted for histamine. All organs examined showed a decrease in histamine, which was greatest in the lung, liver, skin, and heart, in which the levels of histamine were significantly lowered (49.3%, 42.3%, 39.5%, and 32.7% respectively) compared to control values. When tissues were extracted 24 hours after the last dose, the reduction in histamine contents was less. Treatment with compound 48/80 alone for 3 days did not release histamine from guinea pig organs. The administration of 48/80 for 2 weeks reduced the histamine content of the lung by 18.2% and that of the small intestine by 10.7%. This reduction was sustained for at least 7 days, at which time histamine content of the ear was also reduced by 40.2%. Octylamine given for 10 days reduced the histamine content of lung, ear, and small intestine by 34.9%, 28.8%, and.18% respectively. Treatment for 21 days with acetylsalicylic acid increased histamine content of small intestine, ear, and lung by 44.1%, 16.4%, and 10.9% respectively.