USE OF MONOTELOTRISOMICS FOR LINKAGE MAPPING IN BARLEY

Abstract

Analysis of bulk F2 progenies from monotelotrisomic hybrids for two known genes fc and br showed that the gene-centromere distance and frequency of homozygous recessive F2 segregates were inversely related. Based on this relationship the new genes sb, ea, gs3, f5 were assigned to positions on chromosome 1 relative to the previously mapped genes. The centromere position on chromosome 1 was located between the markers rs and n. Two previously undescribed recessive mutants, revoluted leaf (rvl) and Parkland spot (sp,,b), were associated with the long arm of chromosome 5 through the use of monotelotrisomic 14 + 5L.