QUANTITATIVE ISOLATION AND ASSAY OF NEUTRAL GLYCERIDE GLYCEROL AND PHOSPHOGLYCERIDE GLYCEROL-Reference-Cited by-同舟云学术

QUANTITATIVE ISOLATION AND ASSAY OF NEUTRAL GLYCERIDE GLYCEROL AND PHOSPHOGLYCERIDE GLYCEROL

Published:1964-02-01 Issue:2 Volume:42 Page:195-202
ISSN:0008-4018
Container-title:Canadian Journal of Biochemistry
language:en
Short-container-title:Can. J. Biochem.

Author:

DeFreitas A. S. W.,Depocas Florent

Abstract

Neutral glyceride glycerol and phosphoglyceride glycerol obtained by acid-catalyzed methanolysis of 10-100 mg quantities of mixed lipids are separated by paper chromatography. Quantitative elution of the compounds is followed by a periodate oxidation – chromotropic acid assay of the released formaldehyde. Application of this method to the assay of carbon-14-labelled material permits accurate specific activity determinations by radioactivity measurements on phosphoglycerol and on glycerol complexed with sodium borate.

Publisher

Canadian Science Publishing

Subject

General Medicine

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/o64-023

Reference12 articles.

1. Displacement Analysis of Lipids. X. Model Mixtures of Glycerides1

2. THE ISOLATION OF PECTIC SUBSTANCES FROM SOFTWOODS

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